[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
About the Journal
Editorial Board
Keywords
For Authors::
Aims and Scope
Call for Papers
Submission Instructions
For Reviewers::
Referee page
Subscription::
Subscription Form
News & Events::
Journal News
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE
Volume 23, Issue 3 (7-2025)                   Int J Radiat Res 2025, 23(3): 569-576 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yang K, Chen Z. Multislice CT combined with miR-let-7 for colon cancer diagnosis and miR-let-7 targeting HMGA2 to regulate cell cycle alterations in colon cancer cells. Int J Radiat Res 2025; 23 (3) :569-576
URL: http://ijrr.com/article-1-6534-en.html
Multislice CT combined with miR-let-7 for colon cancer diagnosis and miR-let-7 targeting HMGA2 to regulate cell cycle alterations in colon cancer cells
K. Yang , Z. Chen
Department of General Surgery, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha, China , wolf0330cz.edu@hotmail.com
Abstract:   (573 Views)
Background: Colon cancer (CC) is one of the most common malignancies in the digestive tract. In this study, we analysed the diagnostic value of multislice CT (MSCT) in combination with miR-let-7 in CC and the mechanism of action of miR-let-7 in CC. Materials and Methods: A total of 108 patients with suspected CC admitted to our hospital between March 2020 and May 2022 were retrospectively analysed; 37 patients were diagnosed with CC, and the remaining 71 were determined to have benign colonic lesions. To compare the diagnostic value of MSCT, miR-let-7, and MSCT combined with miR-let-7 detection for CC, we detected the expression of miR-let-7 and high mobility group AT hook protein 2 (HMGA2) in normal human colonic epithelial FHC cells and human colonic mucosal epithelial HCT116 cells and observed the effect of miR-let-7 on the biological behaviour of CC cells. Results: Compared with the results of pathological biopsy, the diagnostic sensitivity of MSCT combined with miR-let-7 for CC was 97.30%, the specificity was 98.59%, and the accuracy was 98.15%, with a misdiagnosis rate of only 1.85%. After miR-let-7 expression was silenced, the viability of colon cancer cells increased, and the inflammatory response and oxidative stress increased. In addition, there was a targeted regulatory relationship between HMGA2 and miR-let-7, which could reverse the effect of miR-let-7 on colon cancer cells. Conclusion: The combination of MSCT and the miR-let-7 assay is accurate for the diagnosis of CC, and miR-let-7 regulates the biological behaviour of CC cells through HMGA2.
Keywords: Colonic neoplasms, HMGA2 protein, multidetector computed tomography, inflammation, miR-let-7.
Full-Text [PDF 1017 kb]   (118 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
References
1. Fabregas JC, Ramnaraign B, George TJ (2022) Clinical updates for colon cancer care in 2022. Clinical Colorectal Cancer, 21(3): 198-203. [DOI:10.1016/j.clcc.2022.05.006]
2. Smart N (2023) Colon cancer care is changing and not before time. Colorectal Disease, 25(3): 351. [DOI:10.1111/codi.16545]
3. Jahanafrooz Z, Mosafer J, Akbari M, et al. (2020) Colon cancer therapy by focusing on colon cancer stem cells and their tumor microenvironment. Journal of Cellular Physiology, 235(5): 4153-4166. [DOI:10.1002/jcp.29337]
4. Yalcin S, Philip PA, Athanasiadis I, et al. (2022) Classification of early-stage colon cancer with Immunoscore ((R)): clinical evidence and case studies. Future Oncology, 18(5): 613-623. [DOI:10.2217/fon-2021-0712]
5. Fernandez LM, Parlade AJ, Wasser EJ, et al. (2019) How reliable is CT scan in staging right colon cancer? Diseases of the Colon & Rectum, 62(8): 960-964. [DOI:10.1097/DCR.0000000000001387]
6. Wetterholm E, Rosen R, Rahman M, et al. (2023) CT is unreliable in locoregional staging of early colon cancer: A nationwide registry-based study. Scandinavian Journal of Surgery, 112(1): 33-40. [DOI:10.1177/14574969221132648]
7. Su JL, Chen PS, Johansson G, et al. (2012) Function and regulation of let-7 family microRNAs. Microrna, 1(1): 34-39. [DOI:10.2174/2211536611201010034]
8. You X, Liu M, Liu Q, et al. (2022) miRNA let-7 family regulated by NEAT1 and ARID3A/NF-kappaB inhibits PRRSV-2 replication in vitro and in vivo. PLoS Pathog, 18(10): e1010820. [DOI:10.1371/journal.ppat.1010820]
9. Wang X, Chen Q, Yi S, et al. (2019) The microRNAs let-7 and miR-9 down-regulate the axon-guidance genes Ntn1 and Dcc during peripheral nerve regeneration. Journal of Biological Chemistry, 294(10): 3489-3500. [DOI:10.1074/jbc.RA119.007389]
10. Liu HH, Lee CH, Hsieh YC, et al. (2021) Multiple myeloma driving factor WHSC1 is a transcription target of oncogene HMGA2 that facilitates colon cancer proliferation and metastasis. Biochem Biophys Res Comm, 567: 183-189. [DOI:10.1016/j.bbrc.2021.06.034]
11. Chen CH, Hsieh YC, Yang PM, et al. (2020) Dicoumarol suppresses HMGA2-mediated oncogenic capacities and inhibits cell proliferation by inducing apoptosis in colon cancer. Biochem Biophys Res Comm, 524(4): 1003-1009. [DOI:10.1016/j.bbrc.2020.01.147]
12. Taieb J, Karoui M, Basile D (2021) How I treat stage II colon cancer patients. ESMO Open, 6(4): 100184. [DOI:10.1016/j.esmoop.2021.100184]
13. Obaro AE, Burling DN, Plumb AA (2018) Colon cancer screening with CT colonography: logistics, cost-effectiveness, efficiency and progress. British Journal of Radiology, 91(1090): 20180307. [DOI:10.1259/bjr.20180307]
14. Li Q, Dai P, Zhang W, et al. (2024) Improved diagnostic accuracy of multi-slice spiral CT combined with MRI in colon cancer patients with ileus: A comparative study. Alternative Therapies in Health and Medicine, Published online March 8, 2024.
15. Hong EK, Castagnoli F, Gennaro N, et al. (2021) Locoregional CT staging of colon cancer: does a learning curve exist? Abdominal Radiology (NY), 46(2): 476-485. [DOI:10.1007/s00261-020-02672-7]
16. Hong EK, Landolfi F, Castagnoli F, et al. (2021) CT for lymph node staging of Colon cancer: not only size but also location and number of lymph node count. Abdominal Radiology (NY), 46(9): 4096-4105. [DOI:10.1007/s00261-021-03057-0]
17. Kou N, Liu S, Li X, et al. (2019) H19 facilitates tongue squamous cell carcinoma migration and invasion via sponging miR-let-7. Oncology Research, 27(2): 173-182. [DOI:10.3727/096504018X15202945197589]
18. Liu YX, Bai JX, Li T, et al. (2019) MiR-let-7a/f-CCR7 signalling is involved in the anti-metastatic effects of an herbal formula comprising Sophorae Flos and Lonicerae Japonicae Flos in melanoma. Phytomedicine, 64: 153084. [DOI:10.1016/j.phymed.2019.153084]
19. Joshi S, Wei J, Bishopric NH (2016) A cardiac myocyte-restricted Lin28/let-7 regulatory axis promotes hypoxia-mediated apoptosis by inducing the AKT signalling suppressor PIK3IP1. Biochimica et Biophysica Acta, 1862(2): 240-251. [DOI:10.1016/j.bbadis.2015.12.004]
20. Yin L, Wang Y (2021) Long non-coding RNA NEAT1 facilitates the growth, migration, and invasion of ovarian cancer cells via the let-7 g/MEST/ATGL axis. Cancer Cell International, 21(1): 437. [DOI:10.1186/s12935-021-02018-3]
21. Sun L, He X, Kong J, et al. (2024) Menstrual blood-derived stem cells exosomal miR-let-7 to ameliorate pulmonary fibrosis through inhibiting ferroptosis by Sp3/HDAC2/Nrf2 signalling pathway. International Immunopharmacology, 126: 111316. [DOI:10.1016/j.intimp.2023.111316]
22. Cao L, Zhang Z, Li Y, et al. (2019) LncRNA H19/miR-let-7 axis participates in the regulation of ox-LDL-induced endothelial cell injury via targeting periostin. International Immunopharmacology, 72: 496-503. [DOI:10.1016/j.intimp.2019.04.042]
23. Li Q, Liu W, Chiu PCN, et al. (2020) Mir-let-7a/g enhances uterine receptivity via suppressing Wnt/beta-catenin under the modulation of ovarian hormones. Reproductive Sciences, 27 (5): 1164-1174. [DOI:10.1007/s43032-019-00115-3]
24. Gao X and Wang X (2021) HMGA2 rs968697 T > C polymorphism is associated with the risk of colorectal cancer. Nucleosides Nucleotides Nucleic Acids, 40(8): 821-828. [DOI:10.1080/15257770.2021.1952596]
25. Wu J, Wang Y, Xu X, et al. (2016) Transcriptional activation of FN1 and IL11 by HMGA2 promotes the malignant behaviour of colorectal cancer. Carcinogenesis, 37(5): 511-521. [DOI:10.1093/carcin/bgw029]
26. Xu YZ, Kanagaratham C, Jancik S, et al. (2013) Promoter deletion analysis using a dual-luciferase reporter system. Methods in Molecular Biology, 977: 79-93. [DOI:10.1007/978-1-62703-284-1_7]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
International Journal of Radiation Research
Persian site map - English site map - Created in 0.09 seconds with 50 queries by YEKTAWEB 4722